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Identification of C ulex complex species using SNP markers based on high‐resolution melting analysis

2013; Wiley; Volume: 13; Issue: 3 Linguagem: Inglês

10.1111/1755-0998.12083

1755-0998

David Sung Yong Kang, Cheolho Sim,

Malaria Research and Control

Abstract Mosquitoes belonging to the C ulex pipiens complex are primary vectors for diseases such as West Nile encephalitis, Eastern equine encephalitis, many arboviruses, as well as lymphatic filariases. Despite sharing physiological characteristics, each mosquito species within the C ulex complex has unique behavioural and reproductive traits that necessitate a proper method of identification. Unfortunately, morphometric methods of distinguishing members of this complex have failed to yield consistent results, giving rise to the need for molecular methods of identification. In this study, we propose a novel identification method using high‐resolution melting (HRM) analysis by examining single‐nucleotide polymorphisms in the acetylcholinesterase‐2 ( ace‐2) locus. Our method provides a high confidence for species determination among the three C ulex complex mosquitoes.