Single bacterial cell detection with nonlinear rotational frequency shifts of driven magnetic microspheres
2007; American Institute of Physics; Volume: 91; Issue: 22 Linguagem: Inglês
10.1063/1.2817593
ISSN1520-8842
AutoresBrandon H. McNaughton, Rodney R. Agayan, Roy Clarke, Ron G. Smith, Raoul Kopelman,
Tópico(s)Orbital Angular Momentum in Optics
ResumoShifts in the nonlinear rotational frequency of magnetic microspheres, driven by an external magnetic field, offer a dynamic approach for the detection of single bacterial cells. We demonstrate this capability by optically measuring such frequency shifts when an Escherichia coli attaches to the surface of a 2.0μm magnetic microsphere, thereby affecting the drag of the system. From this change in drag, the nonlinear rotation rate was reduced, on average, by a factor of 3.8. Sequential bacterial cell attachments were also monitored.
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