Glucose oxidase overproduction by the mutant strain M-80.10 of Penicillium variabile in a benchtop fermenter
1997; Elsevier BV; Volume: 21; Issue: 6 Linguagem: Inglês
10.1016/s0141-0229(97)00024-0
ISSN1879-0909
AutoresMaurizio Petruccioli, P. Piccioni, Federico Federici,
Tópico(s)Enzyme Catalysis and Immobilization
ResumoGlucose oxidase production by the mutant M-80.10 and the parental strain of Penicillium variabile required the same nutrient addition to the production medium. Their corresponding batch cultures in a benchtop fermenter resulted in 25.6 and 19.1 U ml−1 of glucose oxidase activity, respectively, thus showing an overproduction of the mutant strain of about one third with respect to the wild type. In both strains, glucose oxidase production was not related to the autolysis phenomena, thus allowing the enzyme under study to be considered as extracellular.
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