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[S]-methionine labelled polypeptides from secondary mouse kidney fibroblasts: Coordinates and one dimensional peptide maps of some major polypeptides

1981; Academic Press; Volume: 5; Issue: 5 Linguagem: Inglês

10.1016/0309-1651(81)90176-4

1878-240X

Stephen J. Fey, Rodrigo Bravo, Paul B. Larsen, Jaime Bellatin, Julio E. Celis,

Peptidase Inhibition and Analysis

A total of 1147 [35S]-methionine labelled polypeptides (826 acidic (IEF) and 321 basic (NEPHGE)) from asynchronous secondary mouse kidney fibroblasts have been separated using high resolution two dimensional gel electrophoresis. Beside numbering the spots, we give for the major polypeptides their coordinates (M.W. and relative mobility respect to beta-actin (IEF) or NEPHGE polypeptide 9 (NEPHGE)). By using one dimensional peptide mapping it has been possible to find homologies between several major mouse and HeLa cell polypeptides.