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Probable Laboratory Contamination of Clinical Specimens with Leptospira meyeri

2003; Wiley; Volume: 47; Issue: 4 Linguagem: Inglês

10.1111/j.1348-0421.2003.tb03400.x

1348-0421

Nobuo Koizumi, Hiroki Kawabata, Haruo Watanabe,

Leptospirosis research and findings

Leptospira meyeri were isolated from the blood or the pleural effusion cultures of four patients at a commercial clinical laboratory. All isolates were identical in their 16S rDNA sequences and NotI or SfiI restriction profiles of the chromosome DNA on pulsed-field gel electrophoresis. However, intraperitoneal inoculation of the isolate (NIID1) failed to kill hamsters and none of the patients' sera reacted with L. meyeri strains isolated, indicating leptospiral contamination occurred during laboratory investigation.