Synthesis of chromogenic substrates of α-amylases on a cyclodextrin basis
1997; Elsevier BV; Volume: 303; Issue: 4 Linguagem: Inglês
10.1016/s0008-6215(97)00187-0
ISSN1873-426X
AutoresErzsébet Farkas, Lóránt Jánossy, János Harangi, Lili Kandra, András Lipták,
Tópico(s)Enzyme Catalysis and Immobilization
ResumoOne-pot acetylation and subsequent partial acetolysis of alpha-, beta- and gamma-cyclodextrins resulted in crystalline peracetylated malto-hexaose, -heptaose, and -octaose, respectively. Prolonged acetolysis of beta-cyclodextrin gave a mixture of acetylated maltooligosaccharides, from which peracetylated malto-triose, -tetraose, and -pentaose were isolated. The acetylated oligosaccharides were converted into alpha-acetobromo derivatives, and then transformed into 4-nitrophenyl and 2-chloro-4-nitrophenyl beta-glycosides. From the 4-nitrophenyl glycosides 4,6-O-benzylidene derivatives were prepared, which were used together with the free glycosides as substrates of porcine pancreatic alpha-amylase.
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