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Inorganic and organic selenium compound speciation with coupled HPLC-MW-HG-AFS

1999; Wiley; Volume: 13; Issue: 10 Linguagem: Inglês

10.1002/(sici)1099-0739(199910)13

1099-0739

José Luis Gómez‐Ariza, Daniel Sánchez-Rodas, E. Morales, O. Herrgott, Iain L. Marr,

Trace Elements in Health

Applied Organometallic ChemistryVolume 13, Issue 10 p. 783-787 Note Inorganic and organic selenium compound speciation with coupled HPLC–MW–HG–AFS J. L. Gómez-Ariza, Corresponding Author J. L. Gómez-Ariza Departamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, SpainDepartamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, Spain.>Search for more papers by this authorD. Sánchez-Rodas, D. Sánchez-Rodas Departamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, SpainSearch for more papers by this authorE. Morales, E. Morales Departamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, SpainSearch for more papers by this authorO. Herrgott, O. Herrgott Chemistry Department, University of Aberdeen, Meston Walk, Old Aberdeen AB9 2UE, Scotland, UKSearch for more papers by this authorI. L. Marr, I. L. Marr Chemistry Department, University of Aberdeen, Meston Walk, Old Aberdeen AB9 2UE, Scotland, UKSearch for more papers by this author J. L. Gómez-Ariza, Corresponding Author J. L. Gómez-Ariza Departamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, SpainDepartamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, Spain.>Search for more papers by this authorD. Sánchez-Rodas, D. Sánchez-Rodas Departamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, SpainSearch for more papers by this authorE. Morales, E. Morales Departamento de Química y Ciencia de los Materiales, Escuela Politécnica Superior, Universidad de Huelva, Campus de La Rábida, Palos de La Frontera, Huelva, SpainSearch for more papers by this authorO. Herrgott, O. Herrgott Chemistry Department, University of Aberdeen, Meston Walk, Old Aberdeen AB9 2UE, Scotland, UKSearch for more papers by this authorI. L. Marr, I. L. Marr Chemistry Department, University of Aberdeen, Meston Walk, Old Aberdeen AB9 2UE, Scotland, UKSearch for more papers by this author First published: 08 September 1999 https://doi.org/10.1002/(SICI)1099-0739(199910)13:10 3.0.CO;2-7Citations: 20AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Coupling between high-performance liquid chromatography, microwave-induced reduction, hydride generation and atomic fluorescence spectrometry (HPLC–MW–HG–AFS) has been used for selenium speciation. The concentrations of a redox mixture (HCl solution of KBr and KBrO3 aqueous solutions) used for both destruction of organic matter and reduction of the selenium species have been optimized, as well as the concentration of NaBH4 in the hydride generation step. Chromatographic separation of selenocysteine, selenite and selenate took place in 15 min, employing a polymer-based strong anion-exchange column. Calibrations with correlation coefficients r > 0.998 were obtained for the three compounds at concentrations between 0.5 and 10 µg l−1. Detection limits for selenite, selenocysteine (SeCys) and selenate were 0.2, 0.3 and 0.5 µg l−1, respectively. The coupling has been tested with spiked water samples (tapwater and seawater), obtaining recoveries in the range 94–104%. Copyright © 1999 John Wiley & Sons, Ltd. Citing Literature Volume13, Issue10Special Issue: Papers from the International Conference on Biological and Environmental Aspects of Main Group Organometallics, Odense, Denmark, June 1998October 1999Pages 783-787 RelatedInformation