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Image analysis of two-dimensional gel electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds

2010; Elsevier BV; Volume: 73; Issue: 8 Linguagem: Inglês

10.1016/j.jprot.2010.01.009

1876-7737

A.R. Brandão, Herbert de Sousa Barbosa, Marco Aurélio Zezzi Arruda,

Transgenic Plants and Applications

Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150-500 microg), the pH separation range (3-10 or 4-7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry.