Assay for the determination of urinary 8-hydroxy-2′-deoxyguanosine by high-performance liquid chromatography with electrochemical detection
1997; Elsevier BV; Volume: 689; Issue: 2 Linguagem: Inglês
10.1016/s0378-4347(96)00328-3
ISSN1872-812X
AutoresDietmar Germadnik, Alexander Pilger, Hugo W. Rüdiger,
Tópico(s)Enterobacteriaceae and Cronobacter Research
ResumoA high-performance liquid chromatographic procedure with electrochemical detection is described for the determination of urinary 8-hydroxy-2'-deoxyguanosine, a major oxidative DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure was followed for extracting 8-hydroxy-2'-deoxyguanosine from human urine and the analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of detection of 8-hydroxy-2'-deoxyguanosine in urine was found to be 0.9 nM. The non-invasive assay provides an indirect measurement of oxidative DNA damage.
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