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Electrolyte transport across the pleura of rabbits

1991; Elsevier BV; Volume: 86; Issue: 1 Linguagem: Inglês

10.1016/0034-5687(91)90044-j

1872-7611

Luciano Zocchi, Emilio Agostoni, D. Cremaschi,

Magnesium in Health and Disease

The amounts of Na+ and Cl in the right pleural space of anesthetized rabbits were determined 10 and 60 min after a 2 ml hydrothorax with the following solutions: Ringer, Ringer with an inhibitor of the Na+ -Cl coupled transport or of the Na+/K+ pump, Ringer with gluconate instead of Cl− or with methylglucamine instead of Na+. During the 10–60 min period: (a) with Ringer Na:su+ and Cl− decreased (P < 0.01) along with an iso-osmotic liquid absorption, (b) with disulfonic-stilbene (0.1 mM), amiloride (0.7 mM), acetazolamide (0.1 mM), or ouabain (0.5 mM) Na+ did not change and Cl− decreased less (P < 0.01) than with Ringer. With gluconate-Ringer or methylglucamine-Ringer the liquid flow reversed: in the former case Cl− and, to a smaller extent, Na+ increased (P < 0.01); in the latter only Na+ increased (P < 0.01). These findings suggest: (1) the occurrence of a Na+/H+ and Cl+t- HCO3− double exchange on the serosal side and of a Na+/K+ pump on the interstitial side of the pleural mesothelium; (2) a slow efflux from the pleural space of gluconate or methylglucamine relative to the corresponding influx of Cl− or Na+, respectively; this drags liquid into the space by osmotic gradient.