Enzyme-linked immunosorbant assay (ELISA) of size-selected crotalid venom antigens by Wyeth's polyvalent antivenom
1988; Elsevier BV; Volume: 26; Issue: 1 Linguagem: Inglês
10.1016/0041-0101(88)90138-9
ISSN1879-3150
AutoresRichard C. Schaeffer, Henry B. Randall, James Resk, Richard W. Carlson,
Tópico(s)Ion channel regulation and function
ResumoR. C. Schaeffer Jr, H. Randall, J. Resk and R. W. Carlson. Enzyme-linked immunosorbant assay (ELISA) of size-selected crotalid venom antigens by Wyeth's polyvalent antivenom. Toxicon26, 67–76, 1988. — The binding of Antivenom (Crotalidae) Polyvalent to fractions from crude venoms of eight crotalid and one viperid snake, obtained by high performance size-exclusion chromatography, was determined with an indirect enzyme-linked immunosorbent assay (ELISA). Most of the large (>30,000 mol. wt) molecular mass crotalid venom fractions were associated with high (>0.7 absorbance units) ELISA values. Similarly, the medium (13,000 – 30,000 mol. wt) and small (<14,000 mol. wt) molecular mass crotalid venom fractions were coincident with moderate (0.3 – 0.7 absorbance units) and low ( 30,000 mol. wt) venom fractions. Thus, antivenom contains minimal levels of antibodies to the basic peptides in these venoms.
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