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Experimental Induction of AGEs in Fetal L132 Lung Cells Changes the Level of Intracellular Cathepsin D

1999; Elsevier BV; Volume: 261; Issue: 1 Linguagem: Inglês

10.1006/bbrc.1999.0902

1090-2104

Michael Kasper, Reinhard Schinzel, Toshimitsi Niwa, Gerald Münch, Martin Witt, Heinz Fehrenbach, Michaela Wilsch‐Bräuninger, Kerstin Pehlke, Andreas Höfer, Richard H. W. Funk,

Neonatal Respiratory Health Research

The effect of the carbonyl compound glyoxal on the induction of advanced glycation end products (AGEs) in the fetal epithelial lung cells L132 was investigated using immunohistochemical, immunoelectron microscopic, and biochemical methods. It was found that glyoxal treatment resulted in morphological changes of the cells and in the membranous and cytosolic localization of AGEs such as methyl-glyoxal-derived compounds, N-(carboxymethyllysine) (CML) and imidazolone. The formation of AGEs was accompanied with a change in the intracellular expression of cathepsin D and a loss of enzymatic activity.