Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells

2011; Multidisciplinary Digital Publishing Institute; Volume: 9; Issue: 4 Linguagem: Inglês

10.3390/md9040603

1660-3397

Leandro Silva Costa, Cinthia Beatrice Silva Telles, Ruth Medeiros Oliveira, Leonardo Thiago Duarte Barreto Nobre, Nednaldo Dantas-Santos, Rafael Barros Gomes Câmara, Mariana Santana Santos Pereira Costa, Jailma Almeida‐Lima, Raniere Fagundes Melo‐Silveira, Ivan Rui Lopes Albuquerque, Edda Lisboa Leite, Hugo Alexandre Oliveira Rocha,

Marine and coastal plant biology

Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated l-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.