Molecular cloning and single-channel properties of the cyclic nucleotide-gated channel from catfish olfactory neurons
1992; Cell Press; Volume: 8; Issue: 1 Linguagem: Inglês
10.1016/0896-6273(92)90107-o
ISSN1097-4199
AutoresEvan H Goulding, John Ngai, Richard Krämer, Suzanne M. Colicos, Richard Axel, Steven A. Siegelbaum, Andrew Chess,
Tópico(s)Biochemical Analysis and Sensing Techniques
ResumoWe have cloned a functional cDNA encoding the cyclic nucleotide-gated channel selectively expressed in cat-fish olfactory sensory neurons. The cyclic nucleotidegated channels share sequence and structural features with the family of voltage-gated ion channels. This homology is most evident in transmembrane region S4, the putative voltage sensor domain, and the H5 domain, thought to form the channel pore. We have characterized the single-channel properties of the cloned catfish channel and compared these properties with the channel in native catfish olfactory sensory neurons. The channel is activated equally well by cAMP and cGMP, shows only a slight voltage dependence of gating, and exhibits a pH-and voltage-dependent subconductance state similar to that observed for the voltage-gated L-type calcium channel.
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