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Detection of 13,19-didesmethyl C spirolide by fluorescence polarization using Torpedo electrocyte membranes

2010; Elsevier BV; Volume: 403; Issue: 1-2 Linguagem: Inglês

10.1016/j.ab.2010.04.006

1096-0309

Eva S. Fonfría, Natalia Vilariño, Jordi Molgó, Rómulo Aráoz, Paz Otero, Begoña Espiña, M. Carmen Louzao, Mercedes Álvarez, Luís M. Botana,

Environmental Toxicology and Ecotoxicology

Fluorescence polarization (FP) is a powerful tool for studying molecular interactions by monitoring changes in the apparent size of fluorescent molecules. In this paper, a previously described fluorescence polarization assay was used to detect 13,19-didesmethyl C spirolide. The assay is based on the competition of cyclic imine marine biotoxins with α-bungarotoxin for binding to nicotinic acetylcholine receptor-enriched membranes of Torpedo marmorata. The 13,19-didesmethyl C spirolide was detected in buffer and mussel matrix. The sensitivity of the assay for the 13,19-didesmethyl C spirolide and the 13-desmethyl C spirolide was similar. After an acetone/chloroform extraction of spiked mussel meat, the average recovery rate of 13,19-didesmethyl C spirolide was 77.7 ± 1.9%. The quantification range for this toxin in mussel was 40–200 μg/kg of shellfish meat. This assay can be used to detect the spirolides 13,19-didesmethyl C spirolide and 13-desmethyl C spirolide, in shellfish as a screening assay.