Spontaneous Immunoglobulin Class Switching in Myeloma and Hybridoma Cell Lines Differs from Physiological Class Switching
1982; Wiley; Volume: 67; Issue: 1 Linguagem: Inglês
10.1111/j.1600-065x.1982.tb01055.x
ISSN1600-065X
AutoresFred Sablitzky, Andreas Radbruch, Klaus Rajewsky,
Tópico(s)Cell Adhesion Molecules Research
ResumoImmunological ReviewsVolume 67, Issue 1 p. 59-72 Spontaneous Immunoglobulin Class Switching in Myeloma and Hybridoma Cell Lines Differs from Physiological Class Switching Fred Sablitzky, Fred Sablitzky Institute for Genetics, University of Cologne, Weyertal 121, D-5000 Koln41, Federal Republic of Germany.Search for more papers by this authorAndreas Radbruch, Andreas Radbruch Institute for Genetics, University of Cologne, Weyertal 121, D-5000 Koln41, Federal Republic of Germany.Search for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal 121, D-5000 Koln41, Federal Republic of Germany.Search for more papers by this author Fred Sablitzky, Fred Sablitzky Institute for Genetics, University of Cologne, Weyertal 121, D-5000 Koln41, Federal Republic of Germany.Search for more papers by this authorAndreas Radbruch, Andreas Radbruch Institute for Genetics, University of Cologne, Weyertal 121, D-5000 Koln41, Federal Republic of Germany.Search for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Weyertal 121, D-5000 Koln41, Federal Republic of Germany.Search for more papers by this author First published: October 1982 https://doi.org/10.1111/j.1600-065X.1982.tb01055.xCitations: 35AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES Baumhäckel, H., Liesegang, B., Radbruch, A., Rajewsky, K. & Sablitzky, F. (1982) Switch from NIP specific IgG3 loIgG1 in the mouse hybridoma cell lineS24/63/63. J. Immunol 128, 1227. Beyreuther, K., Bovens, J., Dildrop, R., Dorff, H., Geske, T., Liesegang, B., Müller, C., Neuberger, M., Radbruch, A., Rajewsky, K., Sablitzky, F., Schreier, P. H. & Zaiss, S. (1981) Isolation and characterisation of class switch variants of myeloma and hybridoma cells. In Immunoglobulin Idiotypes (ICN-UCLA Symposia on Molecular and Cellular Biology), eds. C. Janeway, E. E. Sercarz & H. Wigzell, Vol. XX, p. 229. Academic Press, New York . Bothwell, A. L. M., Paskind, M., Reth, M., Imanishi-Kari, T., Rajewsky, K. & Baltimore, D. (1981) Heavy chain variable region contribution to the NPb family of antibodies: Somatic mutation evident in a γ2a variable region. Cell 24, 625. Burrows, P. D., Beck, G. B. & Wabl, M. R. (1981) Expression of μ and γ immunoglobulin heavy chains in different cells of a cloned lymphoid line. Proc. Natl. Acad. Sci. USA 78, 564. Cory, S., Jackson, J. & Adams, J. (1980) Deletions in the constant region locus can account for switches in immunoglobulin heavy chain expression. Nature 285, 450. Cory, S., Webb, E., Gough, J. & Adams, J. (1981) Recombination events near the immunoglobulin Cμ gene join variable and constant region genes, switch heavy chain expression, or inactivate the locus. Biochemistry 20, 2662. Davis, M., Calame, K., Early, P., Livant, D., Joho, R., Weissmann, I. & Hood, L. (1980a) An immunoglobulin heavy chain gene is formed by at least two recombinational events. Nature 283, 733. Davis, M., Kim, S. & Hood, L. (1980b) DNA sequences mediating class switching in a-immunoglobulins. Science 209, 1360. Dunnick, W., Rabbitts, T. H. & Milstein, C. (1980) An immunoglobulin deletion mutant with implications for the heavy-chain switch and RNA splicing. Nature 286, 669. Francus, T., Dharmgrongartama, B., Campbell, R., Scharff, M. D. & Birshtein, B. (1978) IgG2a-producing variants of an lgG2b-producing mouse myeloma cell line. J. exp. Med. 147, 1535. Gearhart, P. J. & Cebra, J. J. (1981) Most B-cells that have switched surface immunoglobulin isotypes generate clones of cells that do not secrete IgM. J. Immunol. 127, 1030. Gearhart, P. J., Hurwitz, J. L. & Cebra, J. J. (1980) Successive switching of antibody isotypes expressed within the lines of a B-cell clone. Proc. natl. Acad. Sci. USA 77, 5424. Honjo, T. & Kataoka, T. (1978) Organization of immunoglobulin heavy chain genes and allelic deletion model. Proc. natl. Acad. Sci. USA 75, 2140. Hurwitz, J., Coleclough, C. & Cebra, J. J. (1980) CH gene rearrangements in IgM-bearing B cells and in the normal splenic DNA component of hybridomas making different isotypes of antibody. Cell 22, 349. Kataoka, T., Kawakami, T., Takahashi, N. & Honjo, T. (1980) Rearrangement of immunoglobulin γ1-chain gene and mechanism for heavy chain class switch. Proc. natl. Acad Sci. USA 77, 919. Kataoka, T., Miyata, T. & Honjo, T. (1981) Repetitive sequences in class-switch recombination regions of immunoglobulin heavy chain genes. Cell 23, 357. Kenter, A. L. & Birshtein, B. (1979) Genetic mechanism accounting for precise immunoglobulin domain deletion in a variant of MPC 11 myeloma cell line. Science 206, 1307. Lang, R. B., Stanton, W. & Marcu, K. B. (1982) On immunoglobulin heavy chain gene switching: Two γ2h genes are rearranged via switch sequences in MPC 11 cells but only one is expressed. Nucleic Acids Res. 10, 611. Liesegang, B., Radbruch, A. & Rajewsky, K. (1978) Isolation of myeloma variants with predefined variant surface immunoglobulin by cell sorting. Proc. natl. Acad. Sci. USA 75, 3901. Maki, R., Traunecker, A., Sakano, H., Roeder, W. & Tonegawa, S. (1980) Exon shuffling generates an immunoglobulin heavy chain gene. Proc- natl. Acad. Sci. USA 77, 2138. Marcu, K. B., Banerji, J., Penncavage, N., Lang, R., amp; Arnheim, N. (1980) 5′ flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains. Cell 22, 187. Mushinski, J. F., Blattner, F. R., Owens, J. D., Finkelman, F. D., Kessler, S. W. Fitzmaurice, K., Potter, M. & Tucker, P. W. (1980) Mouse immunoglobulin D: construction and characterization of a cloned δ chain cDNA. Proc. natl. Acad. Sci. USA 77, 7405. Neuberger, M. S. & Rajewsky, K. (1981) Switch from hapten-specific immunoglobulin M to immunoglobulin D secretion in a hybrid mouse cell line. Proc. natl. Acad. Sci. USA 78, 1138. Nossal, G. J. V., Szenberg, A., Ada, G. L. & Austin, C. M. (1964) Single cell studies on 19S antibody production. J. exp. med. 119, 485. Obata, M., Kataoka, T., Nakai, S., Yamagishi, H., Takahashi, N., Yamawaki-Kataoka, Y., Nikaido, T., Shimizu, A. & Honjo, T. (1981) Structure of a rearranged γ1 chain gene and its implication to immunoglobulin class-switch mechanism. Proc. natl. Acad. Sci. USA 78, 2437. Perlmutter, A. P. & Gilbert, W. (1982) Gamma immunoglobulin heavy chains are expressed in memory B cells without deletion of Cμ. Proc. natl. Acad. Sci. USA (in press). Rabbitts, T. H., Forster, A., Dunnick, W. & Bentley, D. (1980) The role of gene deletion in the immunoglobulin heavy chain switch. Nature 283, 351. Radbruch, A., Liesegang, B. & Rajewsky, K. (1980) Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class. Proc nail. Acad. Sci. USA 77, 2909. Rogers, J., Choi, E., Souza, L., Carter, C., Word, C., Kuehl, M., Eisenberg, D. & Wall, R. (1981) Gene segments encoding transmembrane carboxy-termini of immunoglobulin γ chains. Cell 26, 19. Sakano, H., Maki, R., Kurusawa, Y., Roeder, W. & Tonegawa, S. (1980) Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature 286, 676. Shimuzu, A., Takahashi, N., Yaoita, Y. & Honjo, T. (1982) Organization of the constant-region gene family of the Mouse immunoglobulin heavy chain. Cell 28, 499. Sitia, R., Rubartelli, A. & Hämmerling, U. (1981) Expression of two immunoglobulin isotypes, IgM and IgA, with identical idiotype in the B cell lymphoma 1.29. J. Immunol 127, 1388. Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol Biol. 98, 503. Wabl, M. R., Forni, L. & Loor, F. (1978) Switch in immunoglobulin class production observed in single clones of committed lymphocytes. Science 199, 1078. Wahl, G., Stern, M. & Stark, G. (1980) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl paper and rapid hybridization using dextran sulfate. Proc. natl. Acad Sci USA 76, 3683. Citing Literature Volume67, Issue1October 1982Pages 59-72 ReferencesRelatedInformation
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