Portal de Periódicos da CAPES

Purification and architecture of the ubiquitous Wave complex

2004; National Academy of Sciences; Volume: 101; Issue: 13 Linguagem: Inglês

10.1073/pnas.0400628101

1091-6490

Alexis Gautreau, Hsin‐Yi Henry Ho, Jiaxu Li, Hanno Steen, Steven P. Gygi, Marc W. Kirschner,

Cell Adhesion Molecules Research

The Wave proteins are major activators of the Arp2/3 complex. The ubiquitous Wave-2 is required for actin polymerization at the leading edge of migrating cells. Here we purify Wave-2 from HeLa cells. Five proteins, Sra, Nap, Wave-2, Abi, and Hspc, are copurified, indicating that they form a tight complex. These proteins are only present in the complexed form, with the exception of Hspc, which displays a free pool. We reconstitute the Wave-2 complex by cotranslating in vitro the five subunits and use this system together with specific immunoprecipitations to study the molecular architecture of the complex. The complex is organized around a core of Nap and Abi. Sra is a peripheral subunit recruited on the Nap side, whereas the Wave and Hspc subunits are recruited on the Abi side of the core.