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Subcutaneous tissue distribution of vancomycin from a fibrin glue/Dacron graft carrier

1997; Wiley; Volume: 36; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-4636(19970915)36

1097-4636

Katsuhiro Fujimoto, Keiko Yamamura, Takashi Osada, Tetsuo Hayashi, Toshitaka Nabeshima, Masahiro Matsushita, Naomichi Nishikimi, Tsunehisa Sakurai, Yuji Nimura,

Wound Healing and Treatments

We investigated the tissue distribution of vancomycin (VCM) incorporated in fibrin glue (FG) in a rat model. One VCM-loaded FG Dacron graft (VCM-FG, VCM 0.6 mg/graft) was implanted in the subcutaneous tissue of the anterior abdominal wall of each rat. VCM was injected intravenously at an equal dose (0.6 mg/rat) after implantation of one control graft (without VCM-FG). After the implantation and the iv injection of an equal dose of VCM (0.6 mg/rat), the tissue distribution of VCM for up to 24 h was determined through analysis of the implanted VCM-FG grafts, which releasd VCM over a 24 h period. The area under the VCM concentration-time curve (AUC) of the tissue was 89.58 μg·h/g after the implantation of the VCM-FG graft, and 7.40 μg·h/g after the iv injection of VCM, respectively. The targeting index of the tissue, defined as the ratio of AUC after the implantation of the VCM-FG graft to that after VCM iv injection, was 12.11. None of the six VCM-FG Dacron grafts after implantation became infected following inoculation with S. aureus ATCC 25923 (0.1 mL 108 CFU/mL). These results suggest that this VCM-FG Dacron graft delivery may be useful in preventing local infection by enhancing the delivery of VCM to the local areas of the implanted site in rats. © 1997 John Wiley & Sons, Inc. J Biomed Mater Res, 36, 564–567, 1997.