Oxylipin analysis methods
2006; Wiley; Volume: 45; Issue: 4 Linguagem: Inglês
10.1111/j.1365-313x.2005.02614.x
ISSN1365-313X
AutoresMartin J. Mueller, Laurent Mène‐Saffrané, Christoph Grun, Kathrin Karg, Edward E. Farmer,
Tópico(s)Hemoglobin structure and function
ResumoSummary Practical guidelines for monitoring and measuring compounds such as jasmonates, ketols, ketodi(tri)enes and hydroxy‐fatty acids as well as detecting the presence of novel oxylipins are presented. Additionally, a protocol for the penetrant analysis of non‐enzymatic lipid oxidation is described. Each of the methods, which employ gas chromatography/mass spectrometry, can be applied without specialist knowledge or recourse to the latest analytical instrumentation. Additional information on oxylipin quantification and novel protocols for preparing oxygen isotope‐labelled internal standards are provided. Four developing areas of research are identified: (i) profiling of the unbound cellular pools of oxylipins; (ii) profiling of esterified oxylipins and/or monitoring of their release from parent lipids; (iii) monitoring of non‐enzymatic lipid oxidation; (iv) analysis of unstable and reactive oxylipins. The methods and protocols presented herein are designed to give technical insights into the first three areas and to provide a platform from which to enter the fourth area.
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