Portal de Periódicos da CAPES

Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring

2013; Microbiology Society; Volume: 63; Issue: Pt_11 Linguagem: Inglês

10.1099/ijs.0.051425-0

1466-5034

Nathaniel A. Losey, Bradley S. Stevenson, Hans‐Jürgen Busse, Jaap S. Sinninghe Damsté, W. Irene C. Rijpstra, Stephen Rudd, Paul A. Lawson,

Metal Extraction and Bioleaching

A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01 T stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50–65 °C, with an optimum at 60 °C, at pH 6.0–8.0, with an optimum at pH 6.5–7.0, and at NaCl concentrations up to 0.5 % (w/v), with optimum growth in the absence of NaCl. Strain MP-01 T was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C 15 : 0 , iso-C 16 : 0 , anteiso-C 17 : 0 and iso-C 17 : 0 . The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, sym- homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01 T represents the first reported cultivated member of subdivision 23 of the Acidobacteria . It is proposed that strain MP-01 T represents a novel genus and species, for which the name Thermoanaerobaculum aquaticum gen. nov., sp. nov. is proposed. The type strain of Thermoanaerobaculum aquaticum is MP-01 T ( = DSM 24856 T = JCM 18256 T ).