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Effect of calcitriol on the secretion of prostaglandin E2, interleukin 1, and tumor necrosis factor α by human monocytes☆

1992; Elsevier BV; Volume: 13; Issue: 2 Linguagem: Inglês

10.1016/8756-3282(92)90010-t

8756-3282

Marı́a T. Zarrabeitia, José A. Riancho, J.A. Amado, José M. Olmos, Jesús González‐Macías,

Advanced Glycation End Products research

Cells of the monocyte/macrophage lineage express specific receptors for calcitriol (1,25-dihydroxyvitamin D3) and secrete prostaglandins and several cytokines with potent effects on bone metabolism. The aim of this study was to determine the effect of calcitriol on the secretion of prostaglandin E2 (PGE2), interleukin-1 (IL-1), and tumor necrosis factor (TNFα). Monocyte-enriched peripheral blood mononuclear cells (PBMC) from healthy subjects were cultured in the presence or absence of calcitriol (10−11 −10−7 M) and several stimulating agents. After 24 h, PGE2, IL-1, and TNFα were measured in the culture supernatants or lysates with specific immunoassays. Calcitriol induced a biphasic effect on PGE2 production by unstimulated cells and increased PGE2 synthesis by cells stimulated with either endotoxin or τ-interferon (IFN-τ). On the other hand, calcitriol inhibited the production of TNFα by monocytes stimulated with either IFN-τ or phorbol esters. This effect was not prevented by the addition of indomethacin, IL-1, or IL-2. Under the conditions used, we observed no effect of calcitriol on IL-1α or IL-1β production. These results indicate that calcitriol induces in vitro marked changes in the secretion of monocyte products with known activity on bone cells. Further studies are needed to elucidate whether some effects of calcitriol on bone metabolism are mediated by the interaction of the sterol with cells of the immune system.