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Application of Sandwich Assay to Resonance Acoustic Microbalance with Naked-Embedded Quartz Biosensor for High-Selectivity Detection of C-Reactive Protein

2013; Institute of Physics; Volume: 52; Issue: 7S Linguagem: Inglês

10.7567/jjap.52.07hd11

1347-4065

Fumihito Kato, Keisuke Tsurimoto, Hirotsugu Ogi, Masahiko Hirao,

Advanced Fiber Optic Sensors

The selectivity of the resonance acoustic microbalance with naked-embedded quartz (RAMNE-Q) biosensor addition to its original features (label-free, high-sensitivity, and replacement-free) was improved by applying a sandwich assay. We succeeded in the real-time monitoring of a frequency change due to a series of reactions: the nonspecific adsorption of streptococcal protein G (SPG), the oriented immobilization of an anti-C-reactive protein (anti-CRP) antibody, the specific binding of CRP as the antigen, and the specific binding of another anti-CRP antibody as the detection antibody. The detection of the low concentrations of CRP (100 pg/ml) and the anti-CRP antibody (1 µg/ml) was carried out repeatedly, and its repeatability was confirmed.