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Determination of the neutral and acidic glycosyl-residue compositions of plant polysaccharides by GC-EI-MS analysis of the trimethylsilyl methyl glycoside derivatives

2001; Elsevier BV; Volume: 46; Issue: 3 Linguagem: Inglês

10.1016/s0144-8617(00)00328-3

1879-1344

Thierry Doco, Malcolm A. O’Neill, Patrice Pellerin,

Microbial Metabolites in Food Biotechnology

A method, using GC-EI-MS analysis of the trimethylsilyl methyl glycoside derivatives, has been developed to identify the neutral and acidic glycosyl-residue compositions of plant polysaccharides. Monosaccharides (15) including four pentoses (arabinose, xylose, 2-O-methylxylose, and apiose), three hexoses (galactose, glucose, and mannose), three 6-deoxy hexoses (rhamnose, fucose, and 2-O-methylfucose), two hexuronic acids (galacturonic and glucuronic acids), two 2-keto-3-deoxy sugars (Kdo and Dha), and aceric acid were quantified. The monosaccharide derivatives were identified by their retention times and by their characteristic GC-MS fragmentation patterns. These monosaccharides are components of plant cell wall polysaccharides and red wine polysaccharides. Thus, GC-MS analysis of trimethylsilyl methyl glycoside derivatives is suitable for determining the neutral and acidic glycosyl-residue compositions of plant cell wall polysaccharides and of polysaccharides present in plant-derived foods and beverages.