Immunochemical determinant of Candida parapsilosis
1983; Elsevier BV; Volume: 117; Linguagem: Inglês
10.1016/0008-6215(83)88089-6
ISSN1873-426X
AutoresMasahiro Funayama, Akemi Nishikawa, Takako Shinoda, Yoshimura Fukazawa,
Tópico(s)Polysaccharides and Plant Cell Walls
ResumoAcetolysis of Candida parapsilosis cell-wall d-mannan, obtained by alkali extraction and purified as a copper complex, gave six oligosaccharides by Bio-Gel P-2 filtration. Their chemical structure was examined by 1H-n.m.r. spectroscopy, methylation analysis, and partial acid hydrolysis. The structures of the penta- and hexa-saccharide were α-d-Manp-(1→3)-α-d-Manp-(1→2)-α-d-Manp-(1→2)-d-Man (M5) and α-d-Manp-(1→2)-α-d-Manp-(1→3)-α-d-Manp-(1→2)-α-d-Manp-(1→2)-α-d-Manp-(1→2)- d-Man (M6), respectively. Inhibition of the oligosaccharides with anti-C. parapsilosis serum and homologous d-mannan indicated that although M5 and M6 exhibited strong inhibitory-activity, M6 was the more effective inhibitor, and suggested that M6 may be the immunodominant or may be responsible for the specificity of C. parapsilosis mannan in the precipitin-reaction system, or both.
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