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High Performance Liquid Chromatographic Assay of Saikosaponins from Radix Bupleuri in China

2005; Pharmaceutical Society of Japan; Volume: 28; Issue: 9 Linguagem: Inglês

10.1248/bpb.28.1736

1347-5215

Xiu-Qin Li, Qiutao Gao, Xiaohong Chen, Kai-Shun Bi,

Traditional Chinese Medicine Analysis

In this study, the quantitative analysis of saikosaponins from Radix Bupleuri in China was performed by high performance liquid chromatography. Saikosaponin-a and -d were converted completely into saikosaponin-b1 and -b2 by mild acid treatment. Distinctive measuring of these converted diene-saponins provided a rapid and selective method for the determination of saikosaponin-a and -d in commercial samples of Radix Bupleuri. The conditions of extraction and conversion of saikosaponins were optimized using orthogonal design L9(3(4)). The HPLC analysis was performed on ODS-C18 column with a flow rate of 1.0 ml/min and detection wavelength of 250 nm. Well resolved chromatograms of saikosaponin-b1 and -b2 were obtained with an isocratic elution of acetonitrile : 1% formic acid water (37.5 : 62.5). Calibration curves of saikosaponin-b1 and -b2 were linear in the range of 4.9-98.0 microg/ml and 3.5-71.0 microg/ml, respectively. The average recovery of saikosaponin-b1 and -b2 were 98.3% (RSD = 3.1%) and 96.4% (RSD = 1.8%), respectively. Seventeen samples of different species and habitats of Radix Bupleuri were analyzed by the developed HPLC method.