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A Nile Red/BODIPY-based bimodal probe sensitive to changes in the micropolarity and microviscosity of the endoplasmic reticulum

2014; Royal Society of Chemistry; Volume: 50; Issue: 79 Linguagem: Inglês

10.1039/c4cc04915b

1364-548X

Zhigang Yang, Yanxia He, Jaehong Lee, Weon‐Sik Chae, Wen Xiu Ren, Joung Hae Lee, Chulhun Kang, Jong Seung Kim,

Advanced biosensing and bioanalysis techniques

We herein report a fluorescent bimodal probe (1) capable of determining ER viscosity and polarity changes using FLIM and fluorescence ratiometry, respectively; during ER stress caused by tunicamycin, the viscosity was increased from ca. 129.5 to 182.0 cP and the polarity of the ER (dielectric constant, ε) enhanced from 18.5 to 21.1.