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Reciprocal recombination products of V K -J K joining reactions in human lymphoid cell lines

1987; Oxford University Press; Volume: 15; Issue: 1 Linguagem: Inglês

10.1093/nar/15.1.1

1362-4962

С. М. Деев, Gabriele Combriato, H.-Gustav Klobeck, Hans G. Zachau,

T-cell and B-cell Immunology

The recombination process that joins a V K to a J K segment of an immunoglobulin gene generates a second, reciprocal recombination product called f fragment. In this second product the regions flanking the V K and J K segments in the germline are joined in a head to head fashion. We now analysed f fragments in the human lymphoid cell lines Daudi, JI and IARC/BL41. All three f fragments contain J K 1 flanks; the V K derived moiety of f Daudi and f41 could be traced back to known germline V K genes. There is a precise head to head joining of the heptanucleotide signal sequences in f Daudi and fJI while in f41 six nucleotides are present between the signal sequences. In contrast to the V K -J K recombination products, the f fragments were found to lack somatic mutations. The structures of the f fragments are discussed in the context of the V K -J K rearrangement mechanism.