On the automated analysis of neutral monosaccharides in glycoproteins and polysaccharides
1972; Elsevier BV; Volume: 21; Issue: 1 Linguagem: Inglês
10.1016/s0008-6215(00)81725-5
ISSN1873-426X
AutoresL. Hough, J.V.S. Jones, Paul Wusteman,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoAn improved method for the automated, quantitative analysis of glycoproteins and polysaccharides for neutral monosaccharide components has been developed, based on the ion-exchange chromatography at pH 7 of sugar-borate complexes. The destruction of sugars during acid hydrolysis has been investigated, and a variety of methods for the neutralization of hydrolysates have been evaluated.
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