Development of a dual fluorogenic and chromogenic dipeptidyl peptidase IV substrate
2006; Elsevier BV; Volume: 16; Issue: 10 Linguagem: Inglês
10.1016/j.bmcl.2006.02.045
ISSN1464-3405
AutoresNan‐Hui Ho, Ralph Weissleder, Ching‐Hsuan Tung,
Tópico(s)Analytical Chemistry and Sensors
ResumoA new far-red dual fluorogenic and chromogenic substrate, 5-glycylprolylglycylprolyl-9-di-3-sulfonyl-propylaminobenza[a]phenoxazonium perchlorate (GPGP-2SBPO), was developed for dipeptidyl peptidase IV (DPP-IV) sensing. The glycylprolylglycylprolyl tetrapeptide was chosen as the recognition sequence due to its stability under physiological conditions. In contrast, the truncated substrate, GP-2SBPO, containing only a glycylprolyl peptide, is unstable. Proteolysis of GPGP-2SBPO was assayed by monitoring the absorbance and fluorescence signals from the released fluorochrome, 2SBPO, at 625 and 670nm, respectively.
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