Tournefortii male sterility system in Brassica napus. Identification, expression and genetic characterization of male fertility restorers
2004; Wiley; Volume: 123; Issue: 5 Linguagem: Inglês
10.1111/j.1439-0523.2004.00960.x
ISSN1439-0523
AutoresRajesh Pahwa, S. S. Banga, K. P. S. Gogna, S. S. Banga,
Tópico(s)Plant biochemistry and biosynthesis
ResumoAbstract A germplasm collection of 152 diverse rapeseed accessions from Canada, China, France, India, Poland and South Korea was assayed for identifying new fertility restorers and sterility maintainers for a Tournefortii (tour) cytoplasmic male sterility (CMS) system in rape‐seed. Only 16 (10.5%) genotypes showed complete fertility restoration following hybridization with tour CMS line NE 409A. Notable among these were GSL 8851, GSL 8953, Mokpo # 9, Mali, Buk‐wuk‐13, Kuju‐27 and Mokpo # 84. As many as 78 (51.3%) genotypes were perfect maintainers of sterility, the remaining 58 (38.2%) genotypes were classified as partial maintainers. To study the inheritance of fertility restoration, 20 CMS ( tour ) rapeseed lines were crossed with the four best fertility restorers, namely GSL 8851, GSL 8953, Kuju‐27 and Mokpo # 9, to obtain F 2 and test cross populations. Segregation data indicated that fertility restoration for tour CMS was governed by two genes, of which, one is stronger than the other (χ 2 12:3:1 ). Differences in gene interactions were also observed (χ 2 9:3:4 ) which could be explained on the basis of influence of female parent genotypes/or modified expression of the restorer gene(s) in different genetic backgrounds. Tests of allelism indicated that the restorer genes present in the four restorers evaluated were allelic.
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