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BIBTEX RIS

Detection of bovine herpesvirus 1 and 5 in semen from Brazilian bulls

2011; Elsevier BV; Volume: 75; Issue: 6 Linguagem: Inglês

10.1016/j.theriogenology.2010.11.025

1879-3231

Martha Trindade Oliveira, Fabrício Souza Campos, Marcelo Maronna Dias, Fernanda Araújo de Britto Velho, Gustavo Eduardo Freneau, Wilia Marta Elsner Diederichsen de Brito, F.A.M. Rijsewijk, Ana Cláudia Franco, Paulo Michel Roehe,

Animal Disease Management and Epidemiology

Bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) are important pathogens of the respiratory and genital tract of cattle and may also affect the central nervous system and cause meningoencephalitis. Both virus types are estimated to be widely distributed in Southern Brazil. In the present study, BoHV-1 and/or BoHV-5 DNA were detected in bovine semen samples from two states of Brazil by two species-specific nested polymerase chain reactions (nPCRs). These nPCRs were used to assay 53 samples of fresh semen and 23 samples of frozen semen from breeding bulls. Viral DNA was detected in all 76 semen samples: all were positive for BoHV-5, whereas 34 of these were positive for BoHV-1 as well. Moreover, in five fresh and in 13 frozen semen samples-of a total number of 40 samples suitable for virus isolation-infectious BoHV-1 and/or BoHV-5 virus were detected. In conclusion, that both BoHV-1 and BoHV-5 were detected in bovine semen in Brazil highlighted the importance of examining bull semen in search for both agents to reduce the risk of transmitting these viruses.