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Coxsackievirus B5 induced apoptosis of HeLa cells: Effects on p53 and SUMO

2009; Elsevier BV; Volume: 396; Issue: 2 Linguagem: Inglês

10.1016/j.virol.2009.10.005

1096-0341

Rogério Gomes, Renata Guerra‐Sá, Eurico Arruda,

Viral Infections and Immunology Research

Coxsackievirus B5 (CVB5), a human enterovirus of the family Picornaviridae, is a frequent cause of acute and chronic human diseases. The pathogenesis of enteroviral infections is not completely understood, and the fate of the CVB5-infected cell has a pivotal role in this process. We have investigated the CVB5-induced apoptosis of HeLa cells and found that it happens by the intrinsic pathway by a mechanism dependent on the ubiquitin–proteasome system, associated with nuclear aggregation of p53. Striking redistribution of both SUMO and UBC9 was noted at 4 h post-infection, simultaneously with a reduction in the levels of the ubiquitin-ligase HDM2. Taken together, these results suggest that CVB5 infection of HeLa cells elicit the intrinsic pathway of apoptosis by MDM2 degradation and p53 activation, destabilizing protein sumoylation, by a mechanism that is dependent on a functional ubiquitin–proteasome system.