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BIBTEX RIS

Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P

2008; Brazilian Chemical Society; Volume: 31; Issue: 7 Linguagem: Inglês

10.1590/s0100-40422008000700043

ISSN

1678-7064

Autores

Omar Mertins, Marcelo Sebben, Paulo H. Schneider, Adriana Raffin Pohlmann, Nádya Pesce da Silveira,

Tópico(s)

Agricultural and Food Sciences

Resumo

PHOSPHATIDYLCHOLINE PURITY BY 1 H AND 31 P NMR.A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of 1 H NMR spectra.Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm.Before purification, a ratio of 19.68±1.37 was determined.Using extraction, a ratio of 10.70±0.61 was found, while from column chromatography, a value of 2.99±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.

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