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Simultaneous Determination of Imipramine, Desipramine, and Their 2-Hydroxy Metabolites in Plasma by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography with Amperometric Detection

1981; Elsevier BV; Volume: 70; Issue: 3 Linguagem: Inglês

10.1002/jps.2600700307

1520-6017

Raymond F. Suckowx, Thomas B. Cooper,

Antibiotics Pharmacokinetics and Efficacy

An ion-pair reversed-phase high-performance liquid chromatographic (HPLC) method, using an electrochemical detector, is presented for the simultaneous and rapid quantitation of imipramine, desipramine, and their 2-hydroxylated metabolites in plasma. The drugs are extracted from 1 ml of plasma at pH 9.7 with ether, back-extracted into 0.1 M HCl, and reextracted into ether following alkalinization. An efficient electrochemical oxidation reaction at the detector electrode affords a low detection level of ~5 ng/ml in a mobile phase of acetonitrile-acetate buffer (40:60) containing 0.005 M heptanesulfonate. Patient data are presented as correlations between the plasma level of each hydroxy metabolite and its respective parent compound. The method is applicable to the laboratory experienced in HPLC.