D-Galactose 6-phosphate: anomeric distribution and analysis of its synthesis from D-galactose and polyphosphoric acid
1981; Elsevier BV; Volume: 88; Issue: 2 Linguagem: Inglês
10.1016/s0008-6215(00)85540-8
ISSN1873-426X
AutoresWilliam C. Wenger, Richard L. Anderson,
Tópico(s)Enzyme function and inhibition
ResumoD-Galactose 6-phosphate as synthesized by direct phosphorylation of D-galactose with polyphosphoric acid is contaminated with two of its positional isomers. These were separated from D-galactose 6-phosphate and from each other, and identified as D-galactose 3- and 5-phosphate by enzymic, chromatographic, and mass-spectral analysis. The previous misidentification of these isomers as furanose forms of D-galactose 6-phosphate has led to erroneous reports concerning the anomeric distribution of D-galactose 6-phosphate. The anomeric distribution of D-galactose 6-phosphate in a purified preparation was determined by gas-liquid chromatography and 13C-n.m.r. spectroscopy to be 32% α-pyranose, 64% β-pyranose, and no more than 4% furanose anomers.
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