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The regulatory effect of histamine on the immune response

1981; Elsevier BV; Volume: 58; Issue: 2 Linguagem: Inglês

10.1016/0008-8749(81)90230-6

1090-2163

Jacques Hébert, R Beaudoin, Micheline Fontaine, Gaétan Fradet,

T-cell and B-cell Immunology

Abstract Suppressive activity on the proliferative response can be generated by preincubating peripheral blood lymphocytes with Con A or histamine. Histamine-induced suppressive activity is weaker than that of Con A and is possibly not mediated by the same T-cell subpopulations. This study compares both suppressive activity on the in vitro IgG production after PWM stimulation as measured with a modified reverse hemolytic PFC assay. Suppressor cells are added to fresh lymphocytes at either the beginning of the culture with PWM or after they have been cultured for 7 days with PWM. In both cases, PFC are performed on Day 7. In such a system, we can confirm previous reports of decreased IgG synthesis by Con A suppressor cells when they are added at the beginning of the culture. The suppression is then 40 ± 12% as compared to 8 ± 9% when suppressor cells are added at the end of the culture. On another hand, histamine suppressor cells lower the IgG production only when added at the end of the culture (43 ± 23 vs 3 ± 3%). These results further substantiate the differences between these two modes of suppression, suggesting that different cells could be involved.