The laboratory diagnosis of periodontal infections
1995; Wiley; Volume: 7; Issue: 1 Linguagem: Inglês
10.1111/j.1600-0757.1995.tb00037.x
ISSN1600-0757
AutoresJoseph J. Zambon, Violet I. Haraszthy,
Tópico(s)Dental Radiography and Imaging
ResumoPeriodontology 2000Volume 7, Issue 1 p. 69-82 The laboratory diagnosis of periodontal infections Joseph J. Zambon, Joseph J. ZambonSearch for more papers by this authorViolet I. Haraszthy, Violet I. HaraszthySearch for more papers by this author Joseph J. Zambon, Joseph J. ZambonSearch for more papers by this authorViolet I. Haraszthy, Violet I. HaraszthySearch for more papers by this author First published: February 1995 https://doi.org/10.1111/j.1600-0757.1995.tb00037.xCitations: 69AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL References 1 Asikainen S, Jousimies-Somer H, Kanervo A, Summanen P. Certain bacterial species and morphotypes in localized juvenile periodontitis and in matched controls. J Periodontol 1987: 58: 224– 230. 2 Beck JD, Koch GG, Zambon JJ, Genco RJ, Tudor GE. Evaluation of oral bacteria as risk indicators for periodontitis in older adults. J Periodontol 1992: 63: 93– 99. 3 Becker W, Becker B, Newman M, Nyman S. Clinical and microbiologic findings that may contribute to dental implant failure. Int J Oral Maxillofac Impl 1990: 5: 31– 38. 4 Bonta Y, Zambon JJ, Neiders M, Genco RJ. Rapid identification of periodontal pathogens in subgingival dental plaque: comparison of indirect immunofluorescence microscopy with bacterial culture for detection of Actinobacillus actinomycetemcomitans. J Dent Res 1985: 64: 793– 798. 5 Bowden G, Johnson J, Schachtele C. Characterization of Actinomyces with genomic DNA fingerprints and rRNA gene probes. J Dent Res 1993: 72: 1171– 1179. 6 Bragd L, Dahlén G, Wikström M, Slots J. The capability of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius to indicate progressive periodontitis; a retrospective study. J Clin Periodontol 1987: 14: 95– 99. 7 Burstain JM, Grimprel E, Lukehart SA, Norgard MV, Radolf JD. Sensitive detection of Treponema pallidum by using the polymerase chain reaction. J Clin Microbiol 1991: 29: 62– 69. 8 Carrassi A, Soligo D, Vogel G, Lambertenghi-Deliliers L, Zambon JJ. Identification of Actinobacillus actinomycetemcomitans by gold immunolabeling and scanning electron microscopy. J Periodontol 1990: 61: 249– 253. 9 Carrassi A, Zambon JJ, Vogel G. A new method of bacterial identification using gold immunolabelling and scanning electron microscopy. Arch Oral Biol 1990: 35: 177S– 180S. 10 Caufield PW, Walker TM. Genetic diversity within Streptococcus mutans evident from chromosomal DNA restriction fragment polymorphisms. J Clin Microbiol 1989: 27: 274– 278. 11 Caugant DA, Selander RK, Olsen I. Differentiation between Actinobacillus (Haemophilus) actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus paraphrophilus by multilocus enzyme electrophoresis. J Gen Microbiol 1990: 136: 2135– 2141. 12 Chen CK, Sunday GJ, Zambon JJ, Wilson ME. Restriction endonuclease analysis of Eikenella corrodens. J Clin Microbiol 1990: 28: 1265– 1270. 13 Chen P, Bochacki V, Reynolds HS et al. The use of monoclonal antibodies to detect Bacteroides gingivalis in biological samples. Infect Immun 1986: 54: 798– 803. 14 Christersson LA, Zambon JJ. Suppression of subgingival Actinobacillus actinomycetemcomitans in localized juvenile periodontitis by systemic tetracycline. J Clin Periodontol 1993: 20: 395– 401. 15 Christersson LA, Albini B, Zambon JJ, Wikesjö UME, Genco RJ. Tissue localization of Actinobacillus actinomycetemcomitans in human periodontitis. I. Light, immunofluorescence and electron microscopic studies. J Periodontol 1987: 58: 529– 539. 16 Christersson LA, Fransson CL, Dunford RG, Zambon JJ. Subgingival distribution of periodontal pathogenic microorganisms in adult periodontitis. J Periodontol 1992: 63: 418– 425. 17 Chuba PJ, Pelz K, Krekeler G, De Isele TS, Gobel U. Synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis. J Gen Microbiol 1988: 134: 1931– 1938. 18 De Buyser M, Morvan A, Grimont F, El Solh N. Characterization of Staphylococcus species by ribosomal RNA gene restriction patterns. J Gen Microbiol 1989: 135: 989– 999. 19 Dix K, Watanabe SM, McArdle S, Lee DI, Randolph C, Moncla B, Schwartz DE. Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. J Clin Microbiol 1990: 28: 319– 323. 20 Drake CW, Hunt J, Beck JD, Zambon JJ. The distribution and interrelationship of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, and BANA scores among older adults. J Periodontol 1993: 64: 82– 94. 21 Dzink JL, Socransky SS, Haffajee AD. The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases. J Clin Periodontol 1988: 15: 316– 323. 22 Ebersole JL, Cappelli D, Steffen MJ. Characteristics and utilization of antibody measurements in clinical studies of periodontal disease. J Periodontol 1992: 63: 1110– 1116. 23 Ebersole JL, Taubman MA, Smith OJ, Haffajee AD. Effect of subgingival scaling on systemic antibody responses to oral microorganisms. Infect Immun 1985: 48: 534– 539. 24 Ebersole JL, Taubman MA, Smith DJ, Hammond BF, Frey DE. Human immune responses to oral microorganisms. II. Serum antibody responses to antigens from Actinobacillus actinomycetemcomitans and the correlation with localized juvenile periodontitis. J Clin Immunol 1983: 3: 321– 331. 25 Ebersole JL, Taubman MA, Smith DJ, Frey DE. Human immune responses to oral microorganisms: patterns of systemic antibody levels to Bacteroides species. Infect Immun 1986: 51: 507– 513. 26 Engleberg NC, Eisenstein BI. Detection of microbial nucleic acids for diagnostic purposes. Annu Rev Med 1992: 43: 147– 55. 27 French CK, Savitt ED, Simon SL et al. DNA probe detection of periodontal pathogens. Oral Microbiol Immunol 1986: 1: 58– 62. 28 Genco RJ, Zambon JJ. Clinical microbiology in the diagnosis and treatment of periodontal disease. J Am Coll Dent 1989: 56: 19– 27. 29 Genco RJ, Zambon JJ, Christersson LA. Use and interpretation of microbiological assays in periodontal diseases. Oral Microbiol Immunol 1986: 1: 73. 30 Gillepsie MJ, Smutko JS, Haraszthy GG, Zambon JJ. Isolation and characterization of the Campylobacter rectus cytotoxin. Microb Pathog 1993: 14: 203– 215. 31 Gobel UB, Paster BJ, Dewhirst FE, Choi BK. Identification of uncultivable spirochetes by PCR amplification of plaque DNA. J Dent Res 1993: 72: 152. 32 Golub LM, Kaplan R, Mulvihill JE, Ramamurthy NS. Collagenolytic activity of crevicular fluid and of adjacent gingival tissue. J Dent Res 1979: 58: 2132– 2136. 33 Griffen AL, Leys EJ, Fuerst PA. Strain identification of Actinobacillus actinomycetemcomitans using the polymerase chain reaction. Oral Microbiol Immunol 1992: 7: 240– 243. 34 Grossi SG, Zambon JJ, Ho AW et al. Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. J Periodontol 1994: 65: 260– 267. 35 Gunaratnam M, Smith GL, Socransky SS, Smith CM, Haffajee AD. Enumeration of subgingival species on primary isolation plates using colony lifts. Oral Microbiol Immunol 1992: 7: 14– 18. 36 Gunsolley JC, Chinchilli VN, Savitt ED et al. Analysis of site specific periodontal bacteria sampling schemes. J Periodontol 1992: 63: 507– 514. 37 Gunsolley JC, Ranney RR, Burmeister JA, Zambon JJ, Schenkein HA. Actinobacillus actinomycetemcomitans in families afflicted with periodontitis. J Periodontol 1990: 61: 643– 648. 38 Haffajee AD, Socransky SS, Ebersole JL, Smith DJ. Clinical, microbiological and immunological features associated with the treatment of active periodontosis lesions. J Clin Periodontol 1984: 11: 600– 618. 39 Haffajee AD, Socransky SS, Dzink JL, Taubman MA, Ebersole JL, Smith DJ. Clinical, microbiological and immunological features of subjects with destructive periodontal diseases. J Clin Periodontol 1988: 15: 240– 246. 40 Hammond BF, Mallonee D. A selective/differential medium for Wolinella recta. J Dent Res 1988: 67: 327. 41 Han N, Hoover CI, Winkler JR, Ng CY, Armitage GC. Identification of genomic clonal types of Actinobacillus actinomycetemcomitans by restriction endonuclease analysis. J Clin Microbiol 1991: 29: 1574– 78. 42 Jolly ME, Wang CH, Ekenberg SJ, Zuelke MS, Kelso DM. Particle concentration fluorescence immunoassay (PCFIA): a new, rapid immunoassay technique with high sensitivity. J Immunol Methods 1984: 67: 21– 35. 43 Kaper JB, Bradford HB, Roberts NC, Falkow S. Molecular epidemiology of Vibrio cholerae in the U.S. gulf coast. J Clin Microbiol 1982: 16: 129– 134. 44 Kuijper EJ, Oudbier JH, Stuifbergen WNHM, Jansz A, Zanen HC. Application of whole-cell DNA restriction endonuclease profiles to the epidemiology of Clostridium difficile-induced diarrhea. J Clin Microbiol 1987: 25: 751– 753. 45 Langenberg W, Rauws EAJ, Widjojokusumo A, Tytgat GNJ, Zanen HC. Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J Clin Microbiol 1986: 24: 414– 417. 46 Laughon BE, Syed SA, Loesche WJ. API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin. J Clin Microbiol 1982: 15: 97– 102. 47 Lee W, Aitken S, Kulkami G et al. Collagenase activity in recurrent periodontitis: relationship to disease progression and doxycycline therapy. J Periodont Res 1991: 26: 479– 485. 48 Listgarten MA, Levin S. Positive correlation between the proportions of subgingival spirochetes and motile bacteria and susceptibility of human subjects to periodontal deterioration. J Clin Periodontol 1981: 8: 122– 138. 49 Listgarten MA. Microbiological testing in the diagnosis of periodontal disease. J Periodontol 1992: 63: 332– 337. 50 Listgarten MA, Lai CH, Evian CI. Comparative antibody titers to Actinobacillus actinomycetemcomitans in juvenile periodontitis, chronic periodontitis and periodontally healthy subjects. J Clin Periodontol 1981: 8: 155. 51 Listgarten MA, Schifter CC, Sullivan P, George C, Rosenberg ES. Failure of a microbial assay to reliably predict disease recurrence in a treated periodontitis population receiving regularly scheduled prophylaxes. J Clin Periodontol 1986: 13: 768– 773. 52 Loesche WJ. The role of spirochetes in periodontal disease. Adv Dent Res 1988: 2: 275– 283. 53 Loesche WJ. DNA probe and enzyme analysis in periodontal diagnostics. J Periodontol 1992: 63: 1102– 1109. 54 Loesche WJ, Bretz WA, Lopatin D et al. Multi-center clinical evaluation of a chairside method for detecting certain periodontopathic bacteria in periodontal disease. J Periodontol 1990: 61: 189– 196. 55 Loesche WJ, Lopatin DE, Giordano J, Alcoforado G, Hujoel P. Comparison of the benzoyl-DL-arginine naphthylamide (BANA) test, DNA probes, and immunological reagents for ability to detect anaerobic periodontal infections due to Porphyromonas gingivalis, Treponema denticola, and Bacteroides forsythus. J Clin Microbiol 1992: 30: 427– 433. 56 Loesche WJ, Syed SA, Stoll J. Trypsin-like activity in subgingival dental plaque: a diagnostic marker for spirochetes and periodontal disease? J Periodontol 1987: 58: 266– 273. 57 Machtei EE, Cho MI, Dunford R, Norderyd J, Zambon JJ, Genco RJ. Clinical, microbiological, and histological factors which influence the success of regenerative periodontal therapy. J Periodontol 1993: 64: 968– 973. 58 Machtei EE, Dunford RG, Norderyd OM, Zambon JJ, Genco RJ. Guided tissue regeneration and anti-infective therapy in the treatment of class II furcation defects. J Periodontol 1993: 64: 968– 973. 59 Mandell RL, Ebersole JL, Socransky SS. Clinical, microbiologic, and immunologic features of active disease sites in juvenile periodontitis. J Clin Periodontol 1987: 14: 534– 540. 60 Mashimo PA, Yamamoto Y, Nakamura M, Slots J. A selective medium for the recovery of Capnocytophaga species from the oral cavity. J Clin Microbiol 1983: 17: 187– 191. 61 McArthur WP, Tsai CC, Boehni PC, Genco RJ, Taichman NS. Leukotoxic effects of Actinobacillus actinomycetemcomitans. Modulation by serum components. J Periodont Res 1981: 16: 159– 170. 62 Mombelli A, Lang NP, Nyman S. Isolation of periodontal species after guided tissue regeneration. J Periodontol 1993: 64: 1171– 1175. 63 Mombelli A, Minder CE, Gusberti FA, Lang NP. Reproducibility of microscopic and cultural data in repeated subgingival plaque samples. J Clin Periodontol 1989: 16: 434– 442. 64 Mombelli A, Van Osten MAC, Schurch E, Lang NP. The microbiota associated with successful or failing osseointegrated titanium implants. Oral Microbiol Immunol 1987: 2: 145– 152. 65 Moncla BJ, Motley ST, Braham P, Ewing L, Adams TH, Vermeulen MJ. Use of synthetic oligonucleotide DNA probes for identification and direct detection of Bacteroides forsythus in plaque samples. J Clin Microbiol 1991: 29: 2158– 2162. 66 Moore LV, Moore WE, Cato EP et al. Bacteriology of human gingivitis. J Dent Res 1987: 66: 989– 995. 67 Newman MG, Socransky SS. Predominant cultivable microbiota in periodontosis. J Periodont Res 1977: 12: 120– 128. 68 Nisengard RJ, Mikulski L, McDuffie DM, Bronson P. Development of a rapid latex agglutination test for periodontal pathogens. J Periodontol 1992: 63: 611– 617. 69 Notten FJW, Nieman FHM, Mikx FHM. Antibiotypes of Bacteroides gingivalis assessed by antimicrobial disks and multivariate analysis. J Clin Microbiol 1985: 22: 1020– 1024. 70 Omar AA, Newman HN, Bulman J, Osborn J. Darkground microscopy of subgingival plaque from the top to the bottom of the periodontal pocket. J Clin Periodontol 1990: 17: 364– 370. 71 Pantera EA, Zambon JJ, Shih-Levine M. Indirect immunofluorescence for the detection of Bacteroides species in human dental pulp. J Endod 1988: 14: 218– 223. 72 Preus HR, Haraszthy VI, Zambon JJ, Genco RJ. Differentiation of Actinobacillus actinomycetemcomitans by arbitrarily primed polymerase chain reaction. J Clin Microbiol 1993: 31: 2773– 2776. 73 Preus HR, Zambon JJ, Machtei EE, Dunford RG, Genco RJ. The distribution and transmission of Actinobacillus actinomycetemcomitans in families with established adult periodontitis. J Periodontol 1994: 65: 2– 7. 74 Reagan DR, Pfaller MA, Hollis RJ, Wenzel RP. Characterization of the sequence of colonization and nosocomial candidemia using DNA fingerprinting and a DNA probe. J Clin Microbiol 1990: 28: 2733– 38. 75 Riviere GR, Wagoner MA, Baker-Zander SA et al. Identification of spirochetes related to Treponema pallidum in necrotizing ulcerative gingivitis and chronic periodontitis. N Engl J Med 1991: 325: 539– 543. 76 Rodenburg JP, Van Winkelhoff AJ, Winkel EG, Goene RJ, Abbas F, De Graaff J. Occurrence of Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in severe periodontitis in relation to age and treatment history. J Clin Periodontol 1990: 17: 392– 399. 77 Rogosa M, Fitzgerald RJ, MacKintosh ME, Beaman AJ. Improved medium for selective isolation of Veillonella. J Bacteriol 1958: 76: 455– 456. 78 Savitt ED, Strzempko MN, Vaccaro KK, Peros WJ, French CK. Comparison of cultural methods and DNA probe analyses for the detection of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, and Bacteroides intermedius in subgingival plaque samples. J Periodontol 1988: 431– 438. 79 Savitt ED, Darack AP, Killoy WJ, Lieberman MG. Site selection criteria for microbiological testing of periodontal microorganisms. J Periodontol 1991: 62: 558– 61. 80 Schenck K. IgG, IgA, and IgM serum antibodies against lipopolysaccharide from Bacteroides gingivalis in periodontal health and disease. J Periodont Res 1985: 20: 368– 377. 81 Scherer S, Stevens DA. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol 1987: 25: 675– 679. 82 Slots J. Selective medium for isolation of Actinobacillus actinomycetemcomitans. J Clin Microbiol 1982: 15: 606– 609. 83 Slots J, Feik D, Rams TE. Actinobacillus actinomycetemcomitans and Bacteroides intermedius in human periodontitis: age relationship and mutual association. J Clin Periodontol 1990: 17: 659– 662. 84 Slots J, Hafström C, Rosling B, Dahlén G. Detection of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in subgingival smears by the indirect fluorescent-antibody technique. J Periodont Res 1985: 20: 613– 6205. 85 Slots J, Reynolds HS, Genco RJ. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun 1980: 29: 1013– 1020. 86 Slots J, Rosling BJ. Suppression of the periodontopathic microflora in localized juvenile periodontitis by systemic tetracycline. J Clin Periodontol 1983: 10: 465– 486. 87 Smith GLF, Socransky SS, Sansome C. "Reverse" DNA hybridization method for the rapid identification of subgingival microorganisms. Oral Microbiol Immunol 1989: 4: 141– 145. 88 Snyder B, Zambon JJ, Reynolds HS, Ryerson CC, Genco RJ. Clinical significance of the Evalusite periodontal test: sensitivity in adult periodontitis. J Dent Res 1994: 73: 305. 89 Socransky SS. Microbiology and periodontal disease: present status and future considerations. J Periodontol 1977: 48: 497. 90 Socransky SS, Haffajee AD. Microbiological risk factors for destructive periodontal disease. In: JD Bader, ed. Risk assessment in dentistry. Chapel Hill : University of North Carolina Dental Ecology, 1990: 79– 90. 91 Strzempko MN, Simon SL, French CK et al. A cross-reactivity study of whole genomic DNA probes for Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis. J Dent Res 1987: 66: 1543– 1546. 92 Taubman MA, Haffajee AD, Socransky SS, Smith DJ, Ebersole JL. Longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases. J Periodont Res 1992: 27: 511– 521. 93 Tew JG, Marshall DR, Moore WEC, Best AM, Palcanis KG, Ranney RR. Serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis. Infect Immun 1985: 48: 303– 311. 94 Van Ketel RJ. Similar DNA restriction endonuclease profiles in strains of Legionella pneumophila from different serogroups. J Clin Microbiol 1988: 26: 1838– 41. 95 Van Ketel RJ, ter Schegget, J, Zanen HC. Molecular epidemiology of Legionella pneumophila serogroup 1. J Clin Microbiol 1984: 20: 362– 364. 96 Van Steenbergen, TJM van der Velden, U, Abbas F, De Graaff J. Microflora and bacterial DNA restriction enzyme analysis in young adults with periodontitis. J Periodontol 1991: 62: 235– 241. 97 Van Steenbergen TJM, Van Winkelhoff, AJ van der Mispel, L van der Velden, U, Abbas F, De Graaff J. Comparison of two selective media for Actinobacillus actinomycetemcomitans. J Clin Microbiol 1986: 24: 636– 638. 98 Van Winkelhoff AJ, Van Steenbergen TJM, De Graaff J. The role of black-pigmented Bacteroides in human oral infections. J Clin Periodontol 1988: 15: 145– 155. 99 Walker CB, Socransky SS. A selective medium for Fusobacterium nucleatum from human periodontal pockets. J Clin Microbiol 1979: 10: 844– 849. 100 Walker CB, Tanner ACR, Smith C, Socransky SS. Selective medium for Eikenella corrodens. J Dent Res 1978: 57: abstr 961. 101 Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S Ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991: 173: 697– 703. 102 Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990: 18: 7213– 7218. 103 White D, Mayrand D. Association of oral Bacteroides with gingivitis and adult periodontitis. J Periodont Res 1981: 16: 259– 261. 104 Wilton JM, Johnson NW, Curtis MA et al. Specific antibody responses to subgingival plaque bacteria as aids to the diagnosis and prognosis of destructive periodontitis. J Clin Periodontol 1991: 18: 1– 15. 105 Wolff LF, Anderson L, Sandberg GP, Aeppli DM, Shelburne CE. A fluorescence immunoassay for detecting periodontal bacterial pathogens in plaque. J Clin Microbiol 1991: 29: 1645– 1651. 106 Wolff LF, Anderson L, Sandberg GP et al. Bacterial concentration fluorescence immunoassay (BCFIA) for the detection of periodontal pathogens in plaque. J Periodontol 1992: 63: 1093– 1101. 107 Wolff LF, Liljemark WF, Bloomquist CG, Pihlstrom BL, Schaffer EM, Bandt CL. The distribution of Actinobacillus actinomycetemcomitans in human plaque. J Periodont Res 1985: 20: 237– 250. 108 Zambon JJ. Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol 1985: 12: 1– 20. 109 Zambon JJ. Microbial risk factors in human periodontal disease. In: JD Bader, ed. Risk assessment in dentistry. Chapel Hill : University of North Carolina Dental Ecology, 1990: 91– 93. 110 Zambon JJ, Grossi S, Dunford R, Haraszthy VI, Preus H, Genco RJ. Epidemiology of subgingival bacterial pathogens in periodontal disease. In: RJ Genco, J McGhee, ed. Molecular pathogenesis of periodontal disease. Washington , DC : Am Soc Microbiol, 1994: 3– 12. 111 Zambon JJ, Reynolds H, Genco RJ. Studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. J Periodontol 1990: 61: 699– 705. 112 Zambon JJ, Reynolds HS, Slots J. Black-pigmented Bacteroides spp. in the human oral cavity. Infect Immun 1981: 32: 198– 203. 113 Zambon JJ, Reynolds HS, Chen P, Genco RJ. Rapid identification of periodontal pathogens in subgingival dental plaque. Comparison of indirect immunofluorescence microscopy with bacterial culture for detection of Bacteroides gingivalis. J Periodontol 1985: suppl: 32– 40. 114 Zambon JJ, Sunday GJ, Smutko JS. Molecular genetic analysis of Actinobacillus actinomycetemcomitans epidemiology. J Periodontol 1990: 61: 75– 80. 115 Zylher L, Jordan H. Development of a partially selective medium for Actinomyces viscosus and A. naeslundii. J Clin Microbiol 1982: 15: 253– 259. 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