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Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea

2003; Korean Society for Parasitology; Volume: 41; Issue: 3 Linguagem: Inglês

10.3347/kjp.2003.41.3.147

1738-0006

Jong-Yil Chai, Aifen Lin, Eun‐Hee Shin, Myoung‐don Oh, Eun‐Taek Han, Ho‐Woo Nam, Soon-Hyung Lee,

Herpesvirus Infections and Treatments

Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a well-known virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea. Key words: Toxoplasma gondii Korean Isolate-1 (KI-1) and RH strain, ocular patient, immunoblotting, culture (in vitro), passage (in mice)