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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

TGF-β1 and BMP-4 carried by liposomes enhance the healing process in alveolar bone

2013; Elsevier BV; Volume: 58; Issue: 6 Linguagem: Inglês

10.1016/j.archoralbio.2012.11.013

ISSN

1879-1506

Autores

Cynthia Lopes Ferreira, Fernando Antônio Mauad de Abreu, Gerluza Aparecida Borges Silva, Frank Ferreira Silveira, Luana Beatriz Araújo Barreto, Tony de Paiva Paulino, Melissa Nunes Miziara, José Bento Alves,

Tópico(s)

Bone and Dental Protein Studies

Resumo

In this work we evaluated the bone-forming potential of BMP4, TGFβ1 and BMP4/TGFβ1 mixed by performing histological and morphometric analysis. We also evaluated the immunolabelling of fibronectin (FN) and collagen type III (Col III), two determinant proteins for the early phase of bone repair. Histological, histomorphometric and immunohistochemistry analysis were used to evaluate new bone and blood vessels formation as well as fibronectin and collagen type III expression. 112 male Wistar rats weighing 250–300 g had their maxillary second molar extracted. Sockets filled with blood clot (BC) or treated with L (empty liposome), P (PBS), BP (BMP-4 in PBS) and TP (TGF-β1 in PBS), as well as with BL (BMP-4 in liposome) and TL (TGF-β1 in liposome) administered isolated or in association (BTL) were obtained. The animals were sacrificed at 3, 7, 14 and 21 days after surgery. An increased percentage of bone trabeculae, and a higher number of blood vessels were observed in groups BL or TL administered isolated or in association when compared to groups BC, L, P, BP and TP. Fibronectin and collagen type III analysis revealed enhanced expression firstly detected at 3 days followed by a peak at 7 days. Lower levels of immunoreactivity were observed in the sockets filled with blood clot, and treated with L, P, BP and TP when compared with sockets from groups BL, TL and BTL. The present study indicates growth factors carried by liposomes, either in isolated or associated forms, as successful enhancers of the healing process in rat tooth sockets. We also conclude that the expression of fibronectin and collagen type III increases during the early phases of bone repair.

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