Taxonomic assessment of Lumbricidae (Oligochaeta) earthworm genera using DNA barcodes
2011; Elsevier BV; Volume: 48; Linguagem: Inglês
10.1016/j.ejsobi.2011.10.003
ISSN1778-3615
AutoresMarcos Pérez‐Losada, R. Howard Bloch, Jesse W. Breinholt, Markus Pfenninger, Jorge Domínguez,
Tópico(s)Study of Mite Species
ResumoThe family Lumbricidae accounts for the most abundant earthworms in grasslands and agricultural ecosystems in the Paleartic region. Therefore, they are commonly used as model organisms in studies of soil ecology, biodiversity, biogeography, evolution, conservation, soil contamination and ecotoxicology. Despite their biological and economic importance, the taxonomic status and evolutionary relationships of several Lumbricidae genera are still under discussion. Previous studies have shown that cytochrome c oxidase I (COI) barcode phylogenies are informative at the intrageneric level. Here we generated 19 new COI barcodes for selected Aporrectodea specimens in Pérez-Losada et al. [1] including nine species and 17 populations, and combined them with all the COI sequences available in Genbank and Briones et al. [2] for Lumbricidae (435 sequences) and seven other Lumbricina families (480 sequences). Our maximum likelihood and Bayesian trees indicate that the genera Aporrectodea, Allolobophora, Eisenia and Dendrobaena (Lumbricidae) and Diplocardia, Metaphire and Amynthas (Megascolecidae) are polyphyletic and so invalid as currently defined. Our results also confirm that COI barcodes are a good proxy for estimating intrageneric phylogenetic diversity and relationships in earthworms.
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