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Effects of incomplete decay in fluorescence lifetime estimation

2011; Optica Publishing Group; Volume: 2; Issue: 9 Linguagem: Inglês

10.1364/boe.2.002517

2156-7085

Regina Leung, Shu‐Chi A. Yeh, Qiyin Fang,

Advanced Optical Sensing Technologies

Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay effect has been shown to lead to overestimation of the amplitude average lifetime except in mono-exponential decays. An inverse model is then developed to correct the error from this effect and the theoretical simulations are tested by experimental results.