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Effect of sodium butyrate on myoblast growth and differentiation

1979; Elsevier BV; Volume: 87; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(79)92042-4

1090-2104

Marie-Pierre Leibovitch, J Kruh,

Biopolymer Synthesis and Applications

Myoblasts from L6 line, after a period of cell division, undergo differentiation into large multinucleated syncitia in 8–9 days. Butyrate was added for 24 hours at various times of culture. In all samples growth was strongly inhibited. After removal of butyrate, growth continued at the same rate for 2 days, afterwards the growth rate became the same as in control cells. Morphological and biochemical differentiations, estimated by creatine phosphokinase assay, occur with a 1–3 day delay according to the time of addition of butyrate, when compared to untreated cells. Only the M form of creatine phosphokinase was present in butyrate-treated cells as in untreated myoblasts.