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BIBTEX RIS

Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice

2010; Elsevier BV; Volume: 28; Issue: 34 Linguagem: Inglês

10.1016/j.vaccine.2010.06.023

ISSN

1873-2518

Autores

Thaís Lourdes Santos Lacerda, Patrícia Gomes Cardoso, Leonardo Augusto de Almeida, Ilana Lopes Baratella da Cunha Camargo, Daniela Almeida Freitas Afonso, Cyntia Cardoso Trant, Gilson Costa Macedo, Eleonora Campos, Silvio Cravero, Suzana P. Salcedo, Jean‐Pierre Gorvel, Sérgio C. Oliveira,

Tópico(s)

Galectins and Cancer Biology

Resumo

Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusDeltawbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-alpha when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. DeltawbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence.

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