Heterodyne detected nonlinear optical imaging in a lock‐in free manner
2012; Wiley; Volume: 5; Issue: 10 Linguagem: Inglês
10.1002/jbio.201200005
ISSN1864-0648
AutoresMikhail N. Slipchenko, Robert A. Oglesbee, Delong Zhang, Wei Wu, Ji‐Xin Cheng,
Tópico(s)Spectroscopy and Chemometric Analyses
ResumoWe report a compact, cost-effective tuned amplifier for frequency-selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock-in detection, as demonstrated through stimulated Raman scattering imaging of live cells and tissues at the speed of 2 μsec/pixel. Application of the tuned amplifier to transient absorption microscopy is also demonstrated. The increased signal to noise ratio allowed epi-detected in vivo imaging of myelin and blood in rat spinal cord with high spatial resolution.
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