The Effect of Ovariectomy and Long-term Estrogen Replacement on Bladder Structure and Function in the Rat
2002; Lippincott Williams & Wilkins; Volume: 168; Issue: 3 Linguagem: Inglês
10.1016/s0022-5347(05)64637-x
ISSN1527-3792
AutoresNicole Fleischmann, George J. Christ, Theresa Sclafani, Arnold Melman,
Tópico(s)Urinary Tract Infections Management
ResumoNo AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Sep 2002The Effect of Ovariectomy and Long-term Estrogen Replacement on Bladder Structure and Function in the Rat Nicole Fleischmann, George Christ, Theresa Sclafani, and Arnold Melman Nicole FleischmannNicole Fleischmann More articles by this author , George ChristGeorge Christ More articles by this author , Theresa SclafaniTheresa Sclafani More articles by this author , and Arnold MelmanArnold Melman More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)64637-XAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The use of estrogen replacement therapy for treating postmenopausal urinary incontinence is a controversial topic. We examined the behavioral, cystometric and histological changes that occur with long-term estrogen depletion and supplementation in rat bladders to determine the role of menopause in lower urinary tract dysfunction. Materials and Methods: A total of 40 female Sprague-Dawley rats were placed into 1 of 3 groups, including bilateral ovariectomy, bilateral ovariectomy plus estrogen replacement and control. The estrogen replaced group received a 0.25 mg. 16-week sustained release pellet (Innovative Research of America, Sanasota, Florida) placed subcutaneously. After surgery voiding frequency and volume were measured in 24-hour periods by placing animals in metabolic cages. After 16 weeks the rats underwent catheterization and continuous cystometry. The bladder was then removed and stained with Gomori trichrome. The collagen-to-smooth muscle density ratio was calculated for each specimen using current imaging software. Results: There was no significant difference in voiding patterns in the 3 groups, as measured by volume and voiding frequency. Cystometric data showed a trend toward higher voiding pressure, threshold pressure, baseline pressure and mean inter-voiding pressure in the ovariectomy group compared with the estrogen and control groups, although there was no statistical significance. Histological studies showed a higher mean collagen-to-smooth muscle ratio plus or minus standard deviation in the ovariectomy group (0.807 ± 0.204) than in the ovariectomy plus estrogen replacement (0.709 ± 0.118) and control (0.700 ± 0.129) groups (p <0.05). Furthermore, when histological and cystometric data were compared for individual samples, we found a direct correlation of mean inter-voiding pressure (a measure of bladder instability) with the collagen-to-smooth muscle ratio (p <0.05). Conclusions: Long-term estrogen replacement is beneficial for treating postmenopausal urinary incontinence. 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Volume 168Issue 3September 2002Page: 1265-1268 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordsestrogen replacement therapybladdermenopauseurinary incontinencerats, Sprague-DawleyMetricsAuthor Information Nicole Fleischmann More articles by this author George Christ More articles by this author Theresa Sclafani More articles by this author Arnold Melman More articles by this author Expand All Advertisement PDF downloadLoading ...
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