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Crystallization and characterization of two crystal forms of the B800–850 light-harvesting complex from Rhodopseudomonas acidophila strain 10050

1989; Elsevier BV; Volume: 209; Issue: 4 Linguagem: Inglês

10.1016/0022-2836(89)90612-8

1089-8638

Miroslav Z. Papiz, Anna M. Hawthornthwaite, Richard J. Cogdell, Kevin J. Woolley, P.A. Wightman, Linda A. Ferguson, J. Gordon Lindsay,

Microbial Community Ecology and Physiology

Two different crystal forms of the B800–850-antenna complex from Rhodopseudomonas acidophila strain 10050 have been grown. This complex is an integral membrane protein and is isolated as an oligomeric assembly with a molecular weight of approximately 84 kDa. This assembly contains six α/β apoprotein pairs, 18 molecules of bacteriochlorophyll a and nine molecules of carotenoid. The first crystal form has dimensions unit cell a = b = 75·8 Å, c = 97·5 Å with the space group P4 and diffracts to a resolution of 12·0 Å. The second crystal form is rhombohedral with dimensions unit cell a = 121·1 Å, α = 60°, space group R32 and diffracts to a resolution of 3·5 Å. Native data have been processes in both cases, to an Rmerge value of 9·0 to 11·0%. The X-ray data suggest that the asymmetric unit, in both crystal forms, contains one 84 kDa antenna complex.