Artigo Revisado por pares

The activation of protein kinase C is not necessary for the monoclonalantibody‐induced modulation of CD3 and CD4 antigens

1990; Wiley; Volume: 20; Issue: 5 Linguagem: Inglês

10.1002/eji.1830200539

ISSN

1521-4141

Autores

Laure Thuillier, Françoise Selz, Philippe Métézeau, Jean‐Louis Pérignon,

Tópico(s)

T-cell and B-cell Immunology

Resumo

We studied the effect of staurosporine, a potent inhibitor of protein kinase C (PKC) activity, on the phorbol ester- or monoclonal antibody (mAb)-induced modulation of CD3 and CD4 surface antigens. Staurosporine (10(-5) M) completely inhibited phorbol ester-induced modulation but had no effect on that induced by mAb. These results indicate that the down-regulation of CD3 and CD4 observed after activation of the cells by the corresponding mAb is independent from PKC-mediated phosphorylations, and thus that the activation of PKC is sufficient but not necessary to induce the modulation of CD3 and CD4 antigens.

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