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Preparation and Characterization of Neurotoxic Tau Oligomers

2010; American Chemical Society; Volume: 49; Issue: 47 Linguagem: Inglês

10.1021/bi1016233

1943-295X

Cristian A. Lasagna‐Reeves, Diana L. Castillo‐Carranza, Marcos J. Guerrero-Muñoz, George R. Jackson, Rakez Kayed,

Neuroinflammation and Neurodegeneration Mechanisms

Tau aggregation is a pathological hallmark of Alzheimer's disease, Parkinson's disease, and many other neurodegenerative disorders known as tauopathies. Tau aggregates take on many forms, and their formation is a multistage process with intermediate stages. Recently, tau oligomers have emerged as the pathogenic species in tauopathies and a possible mediator of amyloid-β toxicity in Alzheimer's disease. Here, we use a novel, physiologically relevant method (oligomer cross-seeding) to prepare homogeneous populations of tau oligomers and characterize these oligomers in vitro. We show that both Aβ and α-synuclein oligomers induce tau aggregation and the formation of β-sheet-rich neurotoxic tau oligomers.