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Molecular Cloning, Chromosomal Location, and Biological Activity of Porcine Interleukin-21

2004; Japanese Society of Veterinary Science; Volume: 66; Issue: 3 Linguagem: Inglês

10.1292/jvms.66.269

1347-7439

Yoshihiro Muneta, Reiko Kikuma, Hirohide Uenishi, Tomoaki Hoshino, Kazuhiro Yoshihara, Maiko Tanaka, Noriyuki Hamashima, Yasuyuki Mori,

Virus-based gene therapy research

A pig interleukin-21 (IL-21) cDNA was successfully cloned and sequenced from porcine peripheral blood lymphocytes (PBL) stimulated with 10 μg/ml concanavalin A (ConA), 10 μg/ml phytohemagglutinin P (PHA), 50 ng/ml phorbol 12-myristate 13-acetate (PMA), and 0.5 μg/ml anti-porcine CD3 antibody for 48 hr. The open reading frame of the porcine IL-21 cDNA is 459 base pairs in length and encodes 152 amino acids. The predicted amino acid sequence of the porcine IL-21 shows 86.2%, 77.7%, and 58.4% identity to the bovine, human, and murine IL-21, respectively. The porcine IL-21 gene was mapped to porcine chromosome 8 (8q22→q23) by means of fluorescence in situ hybridization and radiation hybrid mapping, where the porcine IL-2 gene had been mapped nearby. The recombinant porcine mature IL-21 expressed by E. coli induced dose-dependent proliferation and IFN-γ production from a human NK cell line, NK0. The porcine IL-21 identified in this study will be helpful for the enhancement of innate immune responses of pigs.