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Radioligand binding analysis of knockout mice reveals 5-hydroxytryptamine7 receptor distribution and uncovers 8-hydroxy-2-(di-n-propylamino)tetralin interaction with α2 adrenergic receptors

2004; Elsevier BV; Volume: 124; Issue: 4 Linguagem: Inglês

10.1016/j.neuroscience.2004.01.014

1873-7544

Pascal Bonaventure, Diane Nepomuceno, Lutz Hein, J G Sutcliffe, Timothy W. Lovenberg, Peter B. Hedlund,

Receptor Mechanisms and Signaling

In the present autoradiographic study, we took advantage of 5-hydroxytryptamine7 (5-HT7) receptor knockout mice to analyze the brain distribution of 5-HT7 receptor binding sites using [3H]5-carboxamidotryptamine (5-CT; a 5-HT1A/1B/1D/5/7 receptor ligand) and [3H]8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT; a 5-HT1A/7 receptor ligand). Low to moderate densities of [3H]5-CT (2 nM) binding sites insensitive to pindolol (10 μM, for 5-HT1A/1B receptor blockade) and GR-127935 (1 μM; for 5-HT1D receptor blockade) were observed in wild-type mice (mainly in thalamus and hypothalamus) but not in 5-HT7 receptor knockout mice. Surprisingly, moderate to high densities of [3H]8-OH-DPAT (10 nM) binding sites insensitive to pindolol (10 μM) remained in 5-HT7 receptor knockout mouse brain. These non-5-HT1A, non-5-HT7 binding sites were found to be adrenergic α2A receptor binding sites. In α2A receptor knockout mice low to moderate densities of [3H]8-OH-DPAT binding sites insensitive to pindolol but sensitive to the selective 5-HT7 receptor antagonist SB-269970 (300 nM) were observed mainly in thalamus and hypothalamus. Therefore, in addition to 5-HT1A and 5-HT7 binding sites, [3H]8-OH-DPAT also binds to α2A receptor binding sites in wild-type mouse brain. [3H]8-OH-DPAT (in the presence of pindolol and 1 μM RX-821002 for α2 receptor blockade) and [3H]5-CT (in the presence of pindolol and GR-127935) bind to a similar receptor binding population corresponding to 5-HT7 binding sites. Detailed anatomical mapping of 5-HT7 receptor binding sites in wild-type mouse brain was then performed using both radioligands in the presence of suitable pharmacological agents for non-5-HT7 receptor binding sites blockade. The mapping revealed binding sites consistent with the mRNA distribution with the highest densities found in anterior thalamic nuclei.